A Low Percent Ethanol Method for Immobilizing Planarians

A Low Percent Ethanol Method for Immobilizing Planarians

Planarians have recently become a popular model system for the study of adult stem cells, regeneration and polarity. The system is attractive for both undergraduate and graduate research labs, since planarian colonies are low cost and easy to maintain. Also in situ hybridization, immunofluorescence and RNA-interference (RNAi) gene knockdown techniques have been developed for planarian studies. ...

Evaluating Carriers for Immobilizing Saccharomyces cerevisiae for Ethanol Production in a Continuous Column Reactor

We evaluated a more practical and cost-effective immobilization carriers for ethanol production using the yeast Saccharomyces cerevisiae. Three candidate materials-rice hull, rice straw, and sawdust-were tested for their cell-adsorption capacity and operational durability. Derivatizations of rice hull, rice straw, and sawdust with the optimal concentration of 0.5 M of 2-(diethylamino)ethyl chlo...

Method for immobilizing semiconductors and noble metals on solid surfaces

Formaldehyde-based whole-mount in situ hybridization method for planarians.

Whole-mount in situ hybridization (WISH) is a powerful tool for visualizing gene expression patterns in specific cell and tissue types. Each model organism presents its own unique set of challenges for achieving robust and reproducible staining with cellular resolution. Here, we describe a formaldehyde-based WISH method for the freshwater planarian Schmidtea mediterranea developed by systematic...

Planarians

World Health Organization launched the most ambitious and expensive international programme in history to eradicate smallpox once and for all. After many vicissitudes and no little heroism on the part of the workers sent into remote and hostile territories, the objective was accomplished amidst much jubilation. September of 1977 saw the last recorded case of naturally transmitted smallpox on th...